Tuning the Reduction of Graphene Oxide Nanoflakes Differently Affects Neuronal Networks in the Zebrafish

The increasing engineering of biomedical devices and the design drug-delivery platforms enriched by graphene-based components demand careful investigations impact graphene-related materials (GRMs) on nervous system. In addition, enhanced diffusion GRM-based products technologies that might favor dispersion in environment GRMs nanoparticles urgently requires potential neurotoxicity these compounds to be addressed. One challenges providing definite evidence supporting harmful or safe use is addressing variety this family materials, with differing for size chemistry. Such a diversity impairs reaching unique predictive picture effects Here, exploiting thermal reduction graphene oxide nanoflakes (GO) generate different oxygen/carbon ratios, we used high-throughput analysis early-stage zebrafish locomotor behavior investigate if modifications specific GRM chemical property influenced how nanomaterials affect vertebrate sensory-motor neurophysiology—exposing GO downregulated their swimming performance. Conversely, reduced (rGO) treatments boosted activity. We concluded tuning single properties sufficient produce differential system physiology, likely interfering signaling pathways.